FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757411

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757411
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences548546
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT11870.2163902389225334No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11100.2023531299107094No Hit
CTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTTCC8810.1606064031093108No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7460.13599588730935963No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCTAA252.9024843E-453.8398824
ACGCGTA2350.045.9217227
GACGCGT2350.045.87977226
TTACGCA307.1432674E-444.8542943
GTGGTAT5550.043.669891
CTTATAC2700.042.389451
CTCGTAT1800.042.18599339
CGCGTAG2650.042.0417528
TAATGTC504.095531E-641.0121935
TCGTATG2450.040.98584740
CGTATGC2550.040.8668741
GCGTAGT2800.039.84421529
GCCGTCT2600.039.3414146
CGAGACG2750.039.1527123
ATACGGG957.2759576E-1238.97731
CCGTCTT2550.038.8038247
GTATCAA48250.038.580731
ACCGTAC350.001520210238.47111
TGCCGTC2850.038.2668245
TGTCGTA451.0142085E-438.24711638
Produced by FastQC (version 0.11.5)