FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757412

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757412
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences548546
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28480.5191907333204508No Hit
CTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTTCC9990.18211781691963846No Hit
GTATCAACGCAGAGTACGGGCTAGTCCCTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGC7600.1385480889478731No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTACA150.002641230467.031562
TCAGTAC252.9656428E-453.605693
GTGGTAT7350.049.2701571
ATAAACG352.7010072E-547.862223
ACCGAGT350.001032137341.62262361
TACGCGG350.001553820938.300267
GTATCAA55100.037.6692161
GGTATCA26100.034.6873251
TATCAAC61450.033.8157652
TGCGTCA502.2060744E-433.51272610
TGGTATC11550.031.9197942
ATCAACG65250.031.8861453
GCACGTA450.00392455631.70676851
TCAACGC65400.031.5482334
GTTGTAT752.0822026E-631.2956711
CAACGCA67600.030.6289925
AACGCAG67950.030.5782056
ATTAGAG2100.030.3127423
TAACCGT2050.029.68385353
TTAACGG2200.029.45604535
Produced by FastQC (version 0.11.5)