FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757413

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757413
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences683455
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTTCC18100.2648308959624262No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17960.26278248019255107No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGT13770.20147632250843142No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12210.17865111821553722No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7320.10710288168204198No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGACT150.002951986465.1783753
TATCGTA309.414298E-657.1942738
TGCTTTA200.005384112756.00062655
GTTTGTG701.5932152E-552.32093470
CTCTTAT750.052.1655961
GATTATA253.4003664E-452.1426962
GACACAT200.00751432251.47484638
GGGATCC601.5279511E-1049.41889628
TACGCTA200.00910211649.03805524
GTATACG200.00916298448.95541421
CTTATAC4850.047.0565951
TTATACA5150.044.2959822
ATATCAA1850.044.058781
TATACAC5450.043.05363
GTGGTAT9850.042.6991
TCGTATG3250.041.7003340
TGAATCT3600.041.14368434
AGGGGGG7400.040.58407270
TGTGTAT651.8802893E-840.10976812
TACACAT5900.039.769855
Produced by FastQC (version 0.11.5)