FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757415

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757415
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1188869
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTAAACTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT42000.353276937997374No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT34440.2896870891578467No Hit
GTTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAACCTAAGTAGTTCTTATTGTTGGAGCTGTAAAAAACACT30610.2574715969547528No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26120.21970461001170022No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24290.20431182914181462No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA24160.20321835290515608No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21610.18176939595531552No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20980.1764702418853549No Hit
TTATTATAGTACTCCTCCGACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCAT20840.17529265209203032No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20350.1711710878153943No Hit
TTATAGTACTCCTCCGACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCATCTGTCTT20270.17049817936206596No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20220.17007761157873574No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17950.15098383421554437No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC17800.14972213086555372No Hit
GTACTATAATAATGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT17710.14896510885555936No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16630.1398808447356269No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT16240.1366004160256513No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16120.13559105334565877No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15930.133992895769004No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTC15910.13382466865567189No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTTTA15660.13172182973902086No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC15410.12961899082236983No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC15200.12785260613238295No Hit
GTGTATTACTGTGCGAGAGATGCCCAAGGATACTGGGGTGCCCTTGACTA15050.12659090278239235No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14820.1246562909790734No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT14500.12196465716576006No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14250.11986181824910902No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14120.1187683420124505No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT14110.11868422845578445No Hit
ATTATAGTACTCCTCCGACTTTCGGCCCTGGGACCAAAGTGGATATCAAA13970.11750663866245986No Hit
AACCAGGACAGCCTCCTAAACTGCTCATTTACTGGGCATCTACCCGGGAA13760.115740253972473No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13710.11531968618914278No Hit
GTTTATTACTGTCAGCATTATTATAGTACTCCTCCGACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAA13110.11027287278918031No Hit
ACTTAGGTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAACTGCTCATT12720.10699244407920469No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12640.10631953562587637No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTTTACAGCTCCAA12610.10606719495587823No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12480.10497371871921969No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTTTACAGCTCCAACAATAAGAACTA12440.10463726449255553No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT12320.10362790181256303No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG12210.10270265268923658No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC12110.10186151712257617No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12070.101525062895912No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11960.10059981377258553No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11940.10043158665925347No Hit
CTATAATAATGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC11940.10043158665925347No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3000.048.1508331
TATCGCG851.8189894E-1244.6500936
GGTATCA10600.043.154051
TCGCGTA602.868892E-740.32422638
GTATCAA25750.039.001511
TGGTATC3750.038.5060842
AGTATCG1350.038.30844534
ACCGATC3900.037.93056527
CTTATAC4100.037.7489131
ACGAGAC4000.036.96207422
ATCGCGT753.9768565E-836.8460537
CGATCAG4050.036.5380329
TATACAC4250.036.4028133
GTATCGC1051.6370905E-1136.1270135
CCGATCA4100.036.0848528
CGTATGG501.8263554E-434.61192341
CGCGTAT709.575069E-734.57385639
ATCTCCG4300.034.37464510
TATCAAC29300.034.1457562
TACACAT4600.033.633035
Produced by FastQC (version 0.11.5)