FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757420

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757420
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences754089
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG71150.943522581552045No Hit
CTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTTCC11110.14733008968437414No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT8850.046.997971
GTAAACT504.622134E-640.29962523
GTATCAA58100.038.6811451
AGCGGTT901.1740219E-437.30455870
TATCAAC65400.034.1960452
AACGGAC652.1595003E-433.63509869
GCGTTAT800.002964226533.574170
TGGTATC12200.033.5295522
TAGCACC601.6343789E-533.5206574
GGTATCA27050.032.6128851
TCAACGC73100.030.8610174
ATCAACG73200.030.7333873
CAACGCA74300.030.5019825
ACTTACG553.8547133E-430.47332614
AACGCAG74650.030.2671436
CTTAAGT950.006871088428.27292670
ACGCAGA80350.028.2053187
CGCAGAG80800.028.0482318
GCAGAGT81550.027.7902779
CAGAGTA81700.027.73925810
Produced by FastQC (version 0.11.5)