FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757425

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757425
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1741566
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT35400.20326533705871613No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC28200.1619232346060959No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26230.15061157601836508No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24840.1426302534615398No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24630.14142444214000502No Hit
GTAGGAGACAGAGTCACCATCGTTTGCCGGGCAAGTCAGGGCATTAGAAATGATTTAGGCTGGTATCAGCAGAAA24610.14130960296652553No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23340.13401731545057724No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23100.13263924536882324No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22300.1280456784296432No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCGTTT21910.12580631454679295No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20790.11937532083194091No Hit
CTTCAAGGTTCAGCGGCAGTGGATCTGGCACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTG19750.11340368381100686No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT19120.10978624984640262No Hit
ACCTTGAAGGGACCCCAGTTTGTAAACTGGATGCAGCAAAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTG19030.10926947356574486No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18700.1073746272033331No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG18700.1073746272033331No Hit
CAGTTACCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCAT18590.10674301174919583No Hit
ACTTATTACTGTCTACAAGATCACAGTTACCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA18480.10611139629505859No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG18260.10484816538678407No Hit
GTCTACAAGATCACAGTTACCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCAC17450.1001971788608643No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT8400.047.468331
GTATCAA64300.038.8106461
TATCAAC73500.033.850432
CGTATGC2100.032.98402841
ATCAACG76600.032.6150933
TCAACGC77850.032.267984
CAACGCA79450.031.6614065
AACGCAG80800.031.2600026
TCGTATG2400.030.28391840
ACGCAGA86250.029.3618897
CGCAGAG86850.029.1986088
CGAGACG3100.028.83031523
AGAGTAC88000.028.54534111
CAGAGTA90700.027.73347710
CGTAGAG3150.027.30338928
GCAGAGT92900.027.2978679
GGTATCA47450.026.731011
GACGTAG3350.025.66079726
ACGTAGA3400.025.28924627
ACGAGAC3400.025.27254122
Produced by FastQC (version 0.11.5)