FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757426

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757426
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1741566
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT32360.185809782689832No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28270.16232517171327415No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC23520.13505086801189273No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22120.1270121258683277No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21640.12425598570481967No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21230.12190178264848991No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20770.1192604816584614No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG19700.11311658587730812No Hit
GTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTGCCAGATCCACTGCCG18080.10381461282546857No Hit
GTAGGAGACAGAGTCACCATCGTTTGCCGGGCAAGTCAGGGCATTAGAAATGATTTAGGCTGGTATCAGCAGAAA17460.10025459844760405No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT10150.047.5988581
GTATCAA68200.039.7407071
TATCAAC77000.034.9583322
TTACGGT501.808815E-434.66992641
ATCAACG80800.033.4741743
TCAACGC81650.033.255314
CAACGCA83350.032.5779765
AACGCAG83950.032.3479236
CGCAGAG89950.030.2308718
ACGCAGA90450.029.9493087
AGAGTAC91800.029.06120711
CAGAGTA93250.028.79377410
GCAGAGT94950.028.3503849
AGTACGG63100.026.32048213
GAGTACG63700.026.18233712
GGTATCA43450.025.9447541
TACGGGT8600.024.29615615
TACGGGG27850.024.22965615
TAACGGC2750.023.83318736
GTACGGG69850.023.62651614
Produced by FastQC (version 0.11.5)