FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757428

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757428
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1355407
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG69530.512982447338696No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAAGCG400.00906232549.0925970
GTGGTAT10100.041.1053731
GTATCAA74000.040.3213841
TATCAAC86450.034.262572
GGTATCA32800.034.0619131
TCAACGC89150.033.028374
ATCAACG90000.032.7514953
CAACGCA91500.032.150365
AACGCAG93250.031.6228566
ACGCAGA99200.029.6921127
CGCAGAG100300.029.4663038
TGGTATC14700.029.375352
AGAGTAC102400.028.7610511
CAGAGTA103650.028.54649410
GCAGAGT105300.028.0351059
AGTACGG71850.025.86921313
GAGTACG72950.025.66599712
GTACGGG78350.023.16251214
GAGTACT46800.022.99509812
AGTACTT45950.022.97826213
Produced by FastQC (version 0.11.5)