FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757431

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757431
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1988766
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT27710.13933263139052054No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT10350.039.4016531
CTCGTAT6100.039.0509139
TCGTATG7850.038.34180540
CGTATGC8150.038.2784241
GTATCAA75800.038.198291
GGTATCA36500.035.287421
TATACAC10500.033.648423
CTTATAC10000.033.644091
TATGCCG9200.033.27139743
GCCGTCT9600.032.04516646
TATCAAC92050.032.0343972
ATCAACG97050.030.383993
TCAACGC98450.029.9864234
ATCTCCG10950.029.7798910
CAACGCA100900.029.4266555
ATGCCGT10900.029.40796744
ACGAGAC10950.029.16534222
AACGCAG103100.028.699886
CCCACGA11600.028.10977719
GAGACCG11700.027.59586724
Produced by FastQC (version 0.11.5)