FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757435

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757435
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences594683
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT23980.4032400455368659No Hit
CTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTTCC10240.17219257991232303No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8350.14041094162772436No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6200.10425722611878935No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTACGT150.002657374666.9292826
TTATACA4800.047.931492
TATACAC5100.045.7657933
CTTATAC4900.045.607671
TCGTATG5100.042.70077540
CTCGTAT4600.040.42746439
TACACAT5800.040.2423325
ATTAGTA400.0012657639.93112668
CCGTCTT5500.038.63593347
CGTATGC5600.038.4115641
ACACATC6250.037.3448876
CGAGACA6000.037.2933223
CGCTAGG451.2216269E-437.05784613
ATACACA6300.037.0484964
AGGGGGG5950.036.68089370
GAGGCAA6100.035.93194631
TCTCCGA6500.035.40449511
GTATGCC6150.035.10229542
ATGCCGT6250.034.76655244
TCTCGTA5150.034.6252238
Produced by FastQC (version 0.11.5)