FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757436

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757436
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences594683
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG55840.9389876623343866No Hit
CTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTTCC11580.19472559329928718No Hit
GTATCAACGCAGAGTACGGGCTAGTCCCTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGC6310.10610695109831625No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACGG651.4965026E-552.8762770
TCGATAG350.001582787438.1570523
TGTATAC350.001591986938.11207616
CCTAGCG400.001706027737.57827465
GTATCAA66800.034.5844761
TATCAAC73450.031.4400442
ACGGACC450.005484884629.610323
AACGCAG78050.029.4466046
TCAACGC78250.029.4114364
CAACGCA78350.029.3788365
ATCAACG79950.028.915893
CATAGGA954.33487E-728.061322
GTCGTTT500.00767460827.6419840
ACGCAGA83550.027.6277717
CTAGCGC550.00772475527.60465266
AATTCCG855.8098576E-627.4330885
CGCAGAG84450.027.2938948
AGAGTAC85650.027.0281611
GCAGAGT85400.026.951279
GACACAA500.00883998226.85254128
Produced by FastQC (version 0.11.5)