FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757441

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757441
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences820081
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT44930.5478727101347306No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11490.14010811127193534No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATGTCGTATGCCGT11430.1393764762261289No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9160.11169628365978483No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG11400.059.84488770
CGTAAGC200.00716984552.09052741
TGTTTCG200.00804940950.588079
AATCGCG709.094947E-1248.96438236
TATACAC13500.045.216993
CTTATAC13450.045.145351
GGTACGG307.048952E-444.9781461
GTGGTAT9300.044.978141
TTATACA14000.043.8429952
CTCGTAT9300.042.7113739
TCGTATG12150.041.60940640
TCTCGTA9650.041.38320538
TGGTATC10900.039.604122
CGCGTAT702.165325E-839.47485739
CGTATGC13150.038.82031641
ATCTCCG16050.038.6633610
GCCGTCT11650.038.57402846
GTATGCC13550.038.0093842
ATACACA16200.037.8890084
ACGAGAC16100.037.52566522
Produced by FastQC (version 0.11.5)