FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757442

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757442
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences820081
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG125681.5325315426159123No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT10850.047.5392841
TGGTATC12950.039.7961922
TTAGAGC400.001856902336.93738666
GGTATCA33000.035.244881
ATAGCGT450.002546137434.63580768
GTATCAA61650.034.505411
GTCGCTA501.9585415E-434.20111534
CGGGATA400.002908785833.7083817
CGTTTGC552.993183E-431.81714644
TATCAAC69850.030.3804362
TCTAGTC603.9713355E-430.3187463
GCTACGT605.5526104E-428.63216437
ATCAACG74950.028.302833
TCAACGC76000.027.8231964
CAACGCA76600.027.6086275
AACGCAG78550.027.013936
ACGCAGA85000.024.965577
CGCAGAG86400.024.5610378
ATGTTCG901.4826623E-424.2549963
AGAGTAC87700.024.08173811
Produced by FastQC (version 0.11.5)