FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757445

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757445
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences830398
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT24880.29961536516224746No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8820.1062141286467453No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC5250.048.2780721
GTGGTAT6600.046.0836141
TCTCGTA4500.042.02112638
CTCGTAT4950.039.6996739
ATCTCGT4850.038.91710737
GTATCAA52350.038.7331241
ACGAGAC6600.038.4030122
AATCTCG5300.038.14216636
TCGTATG6300.037.32234240
TATGCCG6450.036.7397843
GGTATCA23250.036.3383331
TCCGAGC7000.036.18675213
CCACGAG7050.035.46385220
CGTATGC6800.035.19286741
CTCCGAG7200.035.1815612
ATCTCCG7350.034.9209810
TATACAC7650.034.880133
GCCGTCT6250.034.84057646
TATCAAC60050.034.7627532
TTATACA7400.034.6912962
Produced by FastQC (version 0.11.5)