FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757447

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757447
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences751958
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT58780.7816925945332053No Hit
CTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTTCC19980.26570632934286226No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17650.23472055620127721No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13180.17527574678372995No Hit
GTATCAACGCAGAGTACGGGCTAGTCCCTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGC10060.1337840677271869No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8950.11902260498591677No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT8380.11144239438904832RNA PCR Primer, Index 33 (95% over 22bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTATGC208.170528E-569.5727441
CAATTTA350.003581603862.0677270
AGGGGGG12700.061.0088270
AAGTGTC200.004414450458.8819466
ATACGGG900.058.253311
TAGTATG252.5404373E-455.3142640
TAACAAC301.2422901E-554.5797842
TAGTACG253.3102144E-452.4279821
TAGGACT253.3200154E-452.3965954
CTTATAC13500.051.9425351
TTATACA13850.050.836052
CCTAAAC200.00882230149.42756727
TAGATAC200.00904131349.1218075
TATACAC14700.048.564873
GTGGTAT11000.048.257571
TACACAT16050.044.4799735
TGTAACG307.585816E-444.3122731
ATACACA16200.044.0681234
TCTGCTT13900.043.5475253
CGTATGC15650.043.1217641
Produced by FastQC (version 0.11.5)