FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757451

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757451
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1142622
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT75710.6625988297092127No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20420.17871176994666654No Hit
CTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTTCC15260.13355247842243542No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14100.12340038963016639No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT12920.1130732648242376TruSeq Adapter, Index 1 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC16050.051.672571
CGTATGA352.163204E-549.69939841
AGGGGGG17800.047.73890370
TTATACA17600.047.293232
GTGGTAT11050.046.6060031
TATACAC18400.046.1453743
TCGTATG19400.042.7056340
TCTCGTA16000.041.49236738
CTCGTAT17350.040.9963339
TGCTTGA21100.040.68910255
CGTATGC20650.040.6018841
GCTTGAA21800.039.78285256
CGAGACT21250.039.66789223
GAGACTA21350.039.65614324
TAGGCAT21100.039.54440729
AGACTAG21250.039.2437925
ACTAGGC21150.039.18481427
ACGAGAC21550.039.1071122
TATGCCG21600.038.8069443
GTATGCC21800.038.78748742
Produced by FastQC (version 0.11.5)