FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757452

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757452
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1142622
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG169251.4812422655961464No Hit
CTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTTCC17540.1535065839796538No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT12300.047.5809361
AATCGTG1004.688207E-640.21393670
GTATCAA96600.038.7740331
GGTATCA43550.035.1704061
TATCAAC106950.035.0017662
ATCAACG118450.031.7265643
TCAACGC118750.031.5338924
CAACGCA120450.031.2370685
AACGCAG122200.030.8498176
TGGTATC19750.030.292752
ATTCACG450.005320736729.79725825
CTCATAT1353.660662E-529.78810170
ACGCAGA130050.028.8617157
CGCAGAG130300.028.7037358
CTAAGCG655.547755E-428.63737565
GCAGAGT132250.028.2805049
CAGAGTA133000.028.14615810
AGAGTAC134500.027.80741111
TCCGTAA857.249472E-526.94938767
GGCGTTA951.3132078E-524.7003625
Produced by FastQC (version 0.11.5)