FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005757453

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757453
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26308
Sequences flagged as poor quality0
Sequence length20-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT5302.0145963205108712No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATGTCGTATGCCGT3191.2125589174395621No Hit
CTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTTCC770.2926866352440322No Hit
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT750.28508438497795346Illumina Paired End PCR Primer 2 (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT750.28508438497795346No Hit
GTATCAACGCAGAGTACGGGCTAGTCCCTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGC700.2660787593127566No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT670.2546753839136384No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCGCGTATGCCGT610.23186863311540218No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATGGCGTATGCCGT580.22046525771628403No Hit
ACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTCT540.2052607571841265Illumina Paired End PCR Primer 2 (95% over 24bp)
ATACACATCTCCGAGCCCACGAGACACTGAGCGATGTCGTATGCCGTCTT470.17865288125285084No Hit
CTGAAAGACAGGCTATTTTATTTCAAAAAAAGAAAAAAAAGTGGGCTCTGGGAACAGGGACTAGCCCGTACTCTG410.15584613045461457No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT410.15584613045461457No Hit
CTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGCCTGT370.14064162992245705No Hit
ACACATCTCCGAGCCCACGAGACACTGAGCGATGTCGTATGCCGTCTTCT370.14064162992245705RNA PCR Primer, Index 1 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT350.1330393796563783No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT350.1330393796563783No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGAGCTCGTATGCCGT350.1330393796563783No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT350.1330393796563783Clontech SMART CDS Primer II A (100% over 21bp)
TAATACGACTCACTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTT310.11783487912422076Clontech Universal Primer Mix Long (95% over 22bp)
GTATCAACGCAGAGTACGGGTTCTAATACGACTCACTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGG300.1140337539911814Clontech Universal Primer Mix Long (95% over 23bp)
GTCCCTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGC290.11023262885814201No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT280.10643150372510264No Hit
ATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTTCCCC280.10643150372510264No Hit
GGTATCAACGCAGAGTACGGGTTCTAATACGACTCACTATAGGGAAGTGA270.10263037859206325Clontech Universal Primer Mix Long (95% over 23bp)
GGTATCAACGCAGAGTACGGGCTAGTCCCTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGC270.10263037859206325No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)