FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757454

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757454
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26308
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24909.464801581268055No Hit
CTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTTCC1090.4143226395012924No Hit
GTATCAACGCAGAGTACGGGCTAGTCCCTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGC780.2964877603770716No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG710.2698798844457959No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT510.19385738178500836No Hit
GTATCAACGCAGAGTACGGGTTCTAATACGACTCACTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGG450.1710506309867721No Hit
CTGAAAGACAGGCTATTTTATTTCAAAAAAAGAAAAAAAAGTGGGCTCTGGGAACAGGGACTAGCCCGTACTCTG430.16344838072069331No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT430.16344838072069331No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT420.15964725558765394No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGG370.14064162992245705No Hit
GTCCCTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGC350.1330393796563783No Hit
GGTATCAACGCAGAGTACGGGCTAGTCCCTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGC340.12923825452333892No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT330.12543712939029952No Hit
TAATACGACTCACTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTT280.10643150372510264No Hit
ATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTTCCCC270.10263037859206325No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCGTG100.007836204139.6583470
GTGGTAT455.30963E-849.65631
CGCCAAG200.008890117549.19471731
GTATCAA4200.036.4821741
TACGGGA704.139656E-836.4128215
TATCAAC4550.033.6758582
TCAACGC5100.029.9870284
ATCAACG5150.029.6958923
GGTATCA2300.029.1460861
CAACGCA5150.029.0772275
AACGCAG5200.028.7976386
CGCAGAG5350.027.9902278
GCAGAGT5500.027.2268589
CAGAGTA5550.026.98157110
AGAGTAC5450.026.89203511
ACGCAGA5600.026.7406657
GAGTACT3200.023.89591412
GAGTACG2950.023.76090812
AGTACTT3100.023.63896813
AGTACGG3000.022.3028513
Produced by FastQC (version 0.11.5)