FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757455

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757455
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1377408
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA46550.036.8954121
GGTATCA23400.034.8815731
GTGGTAT8400.034.4144061
CTCGTAT2700.033.22903439
TATCAAC52650.032.5431332
CGTATGC3550.032.1849341
TCTCGTA2500.031.69278738
TCGTATG3650.031.2430840
ACGAGAC3850.030.91326522
TCAACGC57700.029.7538244
ATCAACG58150.029.4651093
CAACGCA58750.029.2220525
AACGCAG61000.028.088466
TGGTATC10300.027.3947962
TTATCGG658.647545E-426.54390339
TCCGAGC4750.026.47913613
CGCCTTA659.3590474E-426.1868627
ACGCAGA66550.025.8453487
CGCAGAG67550.025.3620938
AGAGTAC67750.025.33831611
Produced by FastQC (version 0.11.5)