FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757457

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757457
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2163519
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT83720.3869621667292961No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA68590.3170298019106835No Hit
GTATAATAATTGGCCTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT64130.29641523832238126No Hit
GTCTGGGACAGAGTTCACTCTCACCATCGGCAGCCTGCAGTCTGAAGATT59340.2742753819125231No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC57120.2640143211129646No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC53580.2476520890271821No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG51520.23813056414110528No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT48940.226205547536213No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT48810.2256046746065091No Hit
GAGTTCACTCTCACCATCGGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTATAATAATTGG47410.21913373536354433No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG45780.21159971324494956No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT42460.1962543430402044No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCACCAACTTGGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCACACT42280.19542236513753752No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC42030.19426684027272237No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAC40890.1889976468891653No Hit
CTCCCACACTCCTCATCTTTGGTGCATCCACCAGGGCCACTGGTATCCCA39710.1835435695272378No Hit
GTGTTAGCACCAACTTGGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCACACTCCTCATCTTTGGTGCATCCA38680.17878280708419939No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCACCAACTTGGCCTGGTA37400.17286651977634584No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT37280.1723118678412346No Hit
TTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCCGATGGTGAGAGTGAACTCTGTC36630.1693075031927152No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGT35990.16634935953878843No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC35100.1622356910200465No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGCGGAGGCCAATTATTATACTG34610.1599708622850088No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT32430.14989468546382076No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG31440.14531880699915278No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG30760.14217577936685558No Hit
GTGTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCCAAGTTGGTGCTA30450.1407429285344848No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC29990.13861676278322493No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28460.1315449506105562No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG28250.1305743097241115No Hit
TCTTTGGTGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT28120.12997343679440762No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA26370.1218847627407016No Hit
AGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCT26120.12072923787588645No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT25960.11998970196240477No Hit
GTCAGAGTGTTAGCACCAACTTGGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCACACTCCTCATCTTTGGTG25950.11994348096781215No Hit
GTTTATTACTGTCAGCAGTATAATAATTGGCCTCCGCTCACTTTCGGCGG25360.11721644228684842No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA25350.11717022129225582No Hit
GGATACCAGTGGCCCTGGTGGATGCACCAAAGATGAGGAGTGTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGG25220.11656934836255194No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25110.11606091742203328No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT25020.11564492847069982No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA24950.11532138150855158No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA24660.11398097266536601No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG24640.1138885306761808No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA24630.11384230968158819No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC24410.11282544780055086No Hit
CTCCTCATCTTTGGTGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTT24090.11134637597358747No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCA24060.11120771298980965No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23490.10857311629803112No Hit
CACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACTCTCACCATCGGCAG23340.10787980137914205No Hit
CAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC22720.10501409971440047No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC21690.10025333727136207No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGCGT306.280715E-446.0474337
GGTATCA28300.041.210681
GTATCAA67950.039.187541
CCGTCGT557.0747137E-637.881847
TATCAAC78600.033.9527552
TCAACGC82600.032.433464
ATCAACG82550.032.369783
CAACGCA84050.031.7920975
AACGCAG86200.031.0390326
CGCAGAG92050.029.1384538
ACGCAGA93750.028.5353977
AGAGTAC93950.028.3681311
GCAGAGT96400.027.9319029
TAAGGCG2400.027.2659829
TCGTATG1555.0931703E-1126.7913240
CAGAGTA101900.026.32365610
GTGGTAT17150.024.8744811
CGTATGC1951.8189894E-1224.85592741
AGTACGG67150.024.6846413
GAGTACG67850.024.37928812
Produced by FastQC (version 0.11.5)