FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757458

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757458
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2163519
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT82930.3833107081564802No Hit
GTATAATAATTGGCCTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT60560.279914343252821No Hit
TTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCCGATGGTGAGAGTGAACTCTGTC60500.27963701728526535No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA52540.2428451055895511No Hit
GTCTGGGACAGAGTTCACTCTCACCATCGGCAGCCTGCAGTCTGAAGATT51090.23614306137362326No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT49520.22888636522258415No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG47920.2214910060877672No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC45950.21238547015302386No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC44840.2072549397532446No Hit
GAGTTCACTCTCACCATCGGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTATAATAATTGG42670.19722498392664914No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGCGGAGGCCAATTATTATACTG40320.18636305019738678No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT39560.18285025460834872No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT37520.1734211717114571No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC37220.17203454187367895No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGT35990.16634935953878843No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT34170.15793713852293417No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC34110.15765981255537853No Hit
GTGTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCCAAGTTGGTGCTA31160.1440246191505598No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30280.1399571716264105No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCACCAACTTGGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCACACT30070.13898653073996578No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT29410.13593594509685378No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29120.1345955362536682No Hit
GTGTTAGCACCAACTTGGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCACACTCCTCATCTTTGGTGCATCCA27470.12696907214588823No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC27400.12664552518374No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA27300.12618331523781395No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG27120.12535133733514706No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAC27110.12530511634055444No Hit
CTCCCACACTCCTCATCTTTGGTGCATCCACCAGGGCCACTGGTATCCCA26950.12456558042707275No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA25990.12012836494618259No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCA25620.11841818814625617No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT25400.11740132626521885No Hit
GTTTATTACTGTCAGCAGTATAATAATTGGCCTCCGCTCACTTTCGGCGG25250.11670801134632976No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG25210.11652312736795933No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG25050.11578359145447764No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24990.115506265486922No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCACCAACTTGGCCTGGTA24840.11481295056803291No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA24690.11411963564914383No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA24220.11194724890329136No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG23850.11023707210336493No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC23770.10986730414662409No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC22760.10519898369277089No Hit
AGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCT21960.10150130412536244No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT21860.10103909417943638No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21810.10080798920647334No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA69000.039.8987241
GGTATCA28350.038.7463381
TATCAAC79150.034.5445862
ATCAACG81900.033.2931943
TCAACGC82150.033.0233424
CAACGCA84000.032.3413545
AACGCAG86400.031.603256
ACGCAGA94300.028.7757957
CGCAGAG94750.028.6391328
AGAGTAC95800.028.57590111
GCAGAGT98300.027.6048589
GTGGTAT18250.026.8971881
CGTACGG658.951546E-426.38816513
CAGAGTA103050.026.33243610
TGTTAGA15700.025.28703770
AGTACGG70500.024.47548913
GAGTACG71700.024.4007712
GCGTACG851.5005971E-424.2150212
GAGTACT41800.023.55364412
AGTACTT42800.022.92317813
Produced by FastQC (version 0.11.5)