FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757462

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757462
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences994221
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34500.34700534388229576No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT15670.1576108330039297No Hit
GTCTACAAACTCCGTTCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA11180.11244984766968309No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10280.10339753435101451No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12450.041.612871
GTATCAA28450.039.073551
GTGGTAT3950.037.3500751
TATCAAC31400.035.3865932
TGGTATC4500.032.7702182
TCAACGC33800.032.7609374
ATCAACG34000.032.5698623
CAACGCA34450.032.1492735
TGCGAGA800.003722445832.0512370
AACGCAG35750.031.2726676
GTACCGA450.00475952130.4839236
ACGCAGA37450.029.8530837
CGCAGAG38200.029.2669628
AGAGTAC38850.029.04211811
CAGAGTA39450.028.77427710
ATTCGAT1156.4979494E-427.87063670
AGTACGG25900.025.94861213
GAGTACG26150.025.83556412
TACGGGG14800.024.09877615
GAGTACT18800.023.8966412
Produced by FastQC (version 0.11.5)