FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757463

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757463
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2293359
Sequences flagged as poor quality0
Sequence length20-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27620.12043469862328575No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT26990.11768763634476766No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGTA7100.041.9033827
AGACGCG7250.040.54171425
TCGTATG7500.039.6883840
CGCGTAG7600.039.619928
GGTATCA47250.039.4011541
GTATCAA108000.037.8603551
GCGTAGT8150.037.38152729
GTGGTAT15600.037.2253341
GACGCGT8250.036.87843726
TGGTATC17000.033.945452
TCTCGTA6950.033.7738738
TATCAAC123500.033.593332
CTCGTAT7100.033.5887239
TCAACGC128650.032.3281824
CGAGACG9150.032.107123
ATCAACG129750.032.080423
CTTATAC10450.032.0349461
CGTAGTA9550.031.92733430
CAACGCA132250.031.499815
CGTATGC9600.031.04977841
Produced by FastQC (version 0.11.5)