FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757465

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757465
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2560748
Sequences flagged as poor quality0
Sequence length20-76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32280.12605691774434657No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55850.039.490861
GTATCAA122700.037.8952941
TCGTATG6100.037.50840840
GTGGTAT18200.035.4002151
CTCGTAT6750.034.34524539
CGTATGC6850.033.4601641
ATCTCCG11950.032.7868310
TATCAAC144100.032.7457732
TCTCGTA6950.032.29391538
TCAACGC154700.030.4800194
ACGAGAC12550.030.41144622
ATCAACG155150.030.3916153
CGAGACG12500.030.26461223
CTTATAC14100.030.2208291
CAACGCA158550.029.8043525
AGACGCT13100.029.14772225
TCTCCGA13400.028.98474511
AACGCAG163850.028.8402826
CGCTCAT13500.028.83536328
CCCACGA13250.028.80030319
Produced by FastQC (version 0.11.5)