FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757469

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757469
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74130
Sequences flagged as poor quality0
Sequence length20-76
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT7360.9928503979495481No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3590.48428436530419533No Hit
CTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTTCC3230.4357210306218805No Hit
GTATCAACGCAGAGTACGGGCTAGTCCCTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGC2620.3534331579657358No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2550.3439902873330636No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATGTCGTATGCCGT2330.31431269391609334No Hit
GTCCCTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGC1470.19830028328611898No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1410.19020639417239985No Hit
CTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGCCTGT1310.1767165789828679No Hit
GGTATCAACGCAGAGTACGGGCTAGTCCCTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGC1250.16862268986914877No Hit
ATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT1230.1659247268312424No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1220.16457574531228922No Hit
CTGAAAGACAGGCTATTTTATTTCAAAAAAAGAAAAAAAAGTGGGCTCTGGGAACAGGGACTAGCCCGTACTCTG1140.1537838931606637No Hit
TCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGCCTGTCTTT1030.1389450964521786No Hit
GTATCAACGCAGAGTACGGGTTCTAATACGACTCACTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGG970.13085120733845945No Hit
TTCTAATACGACTCACTATAGGGAAGTGATACCAGCATCGTCTTGATGCC930.12545528126264668No Hit
TCCCTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGCC930.12545528126264668No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT900.12140833670578711No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCGCGTATGCCGT870.11736139214892755No Hit
TAATACGACTCACTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTT870.11736139214892755No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT850.11466342911102118No Hit
ATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTTCCCC850.11466342911102118No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT820.1106164845541616No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG790.10656953999730204No Hit
ACTCACTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCA780.10522055847834884No Hit
CACTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTT770.10387157695939567No Hit
CCCTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGCCT750.10117361392148927No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGGCGG150.001461970777.7241753
AGGAACC150.002546859767.5862338
AGGGGGG2250.066.3246270
CGTCATA201.1651327E-464.68030533
TGTAGAA200.00303352164.6504266
GATACGG150.003298992563.31907728
CGATACG150.003325721763.19037627
GCGATAC150.003334667363.14759426
GGCGATA150.003343630663.1048725
CCAGTAC150.003361611463.0195924
ACTGCTT201.3298402E-462.97704312
CTGTGCT150.003370628762.977049
GAGGCTC150.003370628762.9770420
AGTATTC200.00422145159.48278457
AAGTATT200.00433645959.0808756
TAAGTAT200.004380183358.93155355
ATTAAGT200.004573459358.29312553
CAATTAA200.004788631657.62088451
TTCAATT200.005092599456.7329749
ATTCAAT200.005208129556.41270448
Produced by FastQC (version 0.11.5)