FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757470

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757470
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74130
Sequences flagged as poor quality0
Sequence length20-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29523.982193443949818No Hit
CTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTTCC3970.5355456630244165No Hit
GTATCAACGCAGAGTACGGGCTAGTCCCTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGC3030.40874140024281663No Hit
GTATCAACGCAGAGTACGGGTTCTAATACGACTCACTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGG1540.20774315391879133No Hit
GTCCCTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGC1540.20774315391879133No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1450.1956023202482126No Hit
CTGAAAGACAGGCTATTTTATTTCAAAAAAAGAAAAAAAAGTGGGCTCTGGGAACAGGGACTAGCCCGTACTCTG1370.18481046809658708No Hit
GGTATCAACGCAGAGTACGGGCTAGTCCCTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGC1340.1807635235397275No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1340.1807635235397275No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1290.17401861594496154No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1250.16862268986914877No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1210.16322676379333603No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGG1170.15783083771752326No Hit
GTATCAACGCAGAGTACGGGGATCTAAAAAAAAAAAAAAAAAAAAAAAAA1070.14434102252799136No Hit
GTTCTAATACGACTCACTATAGGGAAGTGATACCAGCATCGTCTTGATGC1000.13489815189531904No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT990.13354917037636585No Hit
ATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTTCCCC950.12815324430055308No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT880.11871037366788074No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG880.11871037366788074No Hit
TAATACGACTCACTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTT880.11871037366788074No Hit
ACTCACTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCA840.11331444759206798No Hit
ATCAACGCAGAGTACGGGCTAGTCCCTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGC800.10791852151625524No Hit
GGTATCAACGCAGAGTACGGGTTCTAATACGACTCACTATAGGGAAGTGA770.10387157695939567No Hit
CTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGCCTGT770.10387157695939567No Hit
GAGTACGGGCTAGTCCCTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGCCTGTCTTTCAGAT760.10252259544044247No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACTT203.3301656E-4112.6454370
AAGCGGG204.7068712E-577.6478954
AAAGCGG150.001501841277.2001653
GGATACT200.001522459676.9045469
TGAAGAT205.555906E-575.09695449
ATGAAGA150.001730048874.548
AACCCAT150.002007481971.7618744
CGGATAC200.002299925869.3202768
AATGCTG150.003133073264.1485330
AAATGCT150.003167237663.9733829
GGTTTGG150.003306678563.2822381
GTTCGGA200.003376658362.92815865
TTCCCGC200.003699368361.4967263
CGCGGTT200.004003514560.28366561
CATGAAG200.0055646455.478147
AAACTGG302.9491514E-453.58547661
TTCGGAT250.00768501551.1205566
TTGCGTG200.0080078350.60529338
CTTGCGT200.00821511250.27950737
AAGATGG358.1138377E-443.6469951
Produced by FastQC (version 0.11.5)