FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757471

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757471
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1251499
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT47590.3802639874262784No Hit
GTGTGTACCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACCC46740.3734721322190429No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTA37140.2967641204667363No Hit
GTAGTAAACAGCCATGTCGTCGACTCTCAGGCTGTTCATTTGCAGATACA36760.2937277616682075No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34260.2737517169410443No Hit
GGTACACACTGGCCGTGGACGCTCGGCCAGGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT34100.27247325007850587No Hit
GTACACACTGGCCGTGGACGCTCGGCCAGGGGACCAAGGTGGAAATCAAA29230.23355991494999195No Hit
GTGATGGAAACACCTACTTGAATTGGTTTCAGCAGAGGCCAGGCCAGTCTCCAAGGCGCCTAATTTATACGGTTT28690.2292450892889247No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28460.22740729317402572No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27140.21685994155808355No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26780.21398339111737205No Hit
GGCCAGGCCAGTCTCCAAGGCGCCTAATTTATACGGTTTCCAACCGGGAC26200.20934894874067017No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25730.20559345233196352No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT25610.2046346021850597No Hit
GTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCACTGATTTCACACTGAA25370.20271690189125202No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25210.20143843502871359No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT25160.2010389141341703No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC24450.19536571743165596No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT23870.1907312750549541No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23670.18913319147678107No Hit
GTTTACTACTGTGCGAGAAACGGCGGGCATAGTGCCGACTACTGGGGCCA23210.18545759924698302No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC22710.18146239030155037No Hit
AGGTACACACTGGCCGTGGACGCTCGGCCAGGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT22110.17666813956703123No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT21950.17538967270449277No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCCTGGCCGAGCGTC21750.17379158912631973No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG21690.1733121640528678No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC21590.17251312226378127No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20790.16612078795108906No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20690.16532174616200251No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19880.15884950767040165No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG19740.15773084916568053No Hit
GTCTGGGACCCCAGAGTCCCGGTTGGAAACCGTATAAATTAGGCGCCTTGGAGACTGGCCTGGCCTCTGCTGAAA19660.1570916157344113No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19550.1562126697664161No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAATCAGTGCCTGACCCACTGCCG19450.1554136279773296No Hit
GGCCGTGGACGCTCGGCCAGGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC19400.15501410708278632No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19020.1519777482842575No Hit
CTCGTACACAGTGATGGAAACACCTACTTGAATTGGTTTCAGCAGAGGCC18490.14774282680209894No Hit
GGCCTGGCCTCTGCTGAAACCAATTCAAGTAGGTGTTTCCATCACTGTGT18200.145425605613748No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC17590.14055145070032019No Hit
TCCACGGCCAGTGTGTACCTTGCATGCAGTAATAAACCCCAACATCCTCA17420.13919307965887306No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17300.13823422951196926No Hit
GCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGT17260.13791461279633466No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA17190.13735528354397408No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17130.13687585847052214No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG16970.1355973916079837No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAGCTGGG16950.13543758325016642No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT16880.13487825399780584No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16400.1310428534101905No Hit
GTTTCCAACCGGGACTCTGGGGTCCCAGACAGATTCAGCGGCAGTGGGTC16400.1310428534101905No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGTCTCCACTCTCT16140.12896534475856553No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA15900.12704764446475786No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15800.12624860267567134No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC15420.12321224387714252No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA15380.12289262716150791No Hit
TTCCATCACTGTGTACGAGGCTTTGACTAGACCTGCAGGAGATGGAGGCC15340.1225730104458733No Hit
GTCTCCACTCTCTCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTACA15320.122413202088056No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15260.12193377701460409No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15260.12193377701460409No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC15230.12169406447787813No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15130.12089502268879161No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT15010.11993617254188776No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14960.11953665164734449No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14810.11833808896371471No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14790.11817828060589741No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG14650.11705962210117626No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGTCTCCACT14490.11578115523863783No Hit
GTCTCCAAGGCGCCTAATTTATACGGTTTCCAACCGGGACTCTGGGGTCC14480.11570125105972917No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14150.11306441315574363No Hit
GCAGTGGGTCAGGCACTGATTTCACACTGAAAATCAGCAGGGTGGAGGCT14110.11274479644010901No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA14060.11234527554556575No Hit
CTCTCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTA13980.11170604211429654No Hit
TTATTACTGCATGCAAGGTACACACTGGCCGTGGACGCTCGGCCAGGGGA13930.11130652121975326No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGA13820.11042757525175809No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC13650.109069204210311No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG13610.10874958749467639No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT13420.107231408095412No Hit
CTCCAGCCCCTGGCCTGGAGCCTGGCGGACCCAGCCCATCCAATAAGTAT13410.10715150391650333No Hit
CTCCAGAGACAACGCCAAGAGCTCACTGTATCTGCAAATGAACAGCCTGAGAGTCGACGACATGGCTGTTTACTA13290.1061926537695995No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13120.1048342827281524No Hit
GGTCTAGTCAAAGCCTCGTACACAGTGATGGAAACACCTACTTGAATTGGTTTCAGCAGAGGCCAGGCCAGTCTC13020.10403524093906588No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12980.10371562422343127No Hit
GTCCAAGGGTGACGGGCAGAGAGAGTGGAGACTGAGTCATCACAACATCC12890.10299648661325339No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA12830.10251706153980146No Hit
GCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGC12790.10219744482416686No Hit
GCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAG12670.10123859467726304No Hit
ATATTATGTGGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACG12630.10091897796162842No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAT309.0167905E-657.6174239
GTGGTAT3100.055.4913251
TGGTATC3100.055.471372
ATCGCGT200.007327923651.80573737
CTTATAC2650.046.7383541
GGTATCA10050.045.5304951
CGTATGC2400.040.39222341
TCGTATG2200.039.31619340
TTATACA3200.038.6912772
TATACAC3300.038.5594643
TAGCGCT3050.038.3879929
CTAGCGC3000.037.8738528
GCCGTCT2200.036.28174246
ATGCCGT2600.035.99986344
ACTAGCG3100.035.53717827
CTCGTAT1950.035.4568839
CGAGACT3050.034.97422423
GTATCAA20750.034.4875221
ACGAGAC3100.034.4018722
TACACAT3700.034.3908735
Produced by FastQC (version 0.11.5)