FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757473

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757473
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3290879
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT40630.12346245486388287No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG13000.038.35685340
GGTATCA49800.038.248711
GTATCAA108100.038.22021
CGTATGC13650.037.84527241
CTCGTAT10100.036.98560739
CTTATAC18600.034.442921
TATCAAC125300.032.9882
TATACAC21000.032.9406243
ACGAGAC19000.032.8132722
TATGCCG15900.032.344443
CCCACGA19300.032.11645519
ATGCCGT16550.031.31203344
TCTCGTA11950.031.22529838
ATCAACG132650.031.21183
TCAACGC133650.030.9526424
CAACGCA135450.030.642445
ATCTCCG20700.030.10884910
GTGGTAT19600.030.063791
TCCGAGC20750.029.87126513
AACGCAG139550.029.717626
Produced by FastQC (version 0.11.5)