FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757475

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757475
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences959262
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGTACTTTTTCCTGGTCGTTCGGCCAAGGGACCAAGGTGGAAGTCAAACGAACTGTGGCTGCACCAT25750.26843552647764635No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT24810.25863632667613223No Hit
GTACTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC19460.2028642852526213No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19050.19859016619025874No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17060.17784505171684067No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16830.17544737516966166No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16440.17138174972009732No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16220.16908831997931745No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC15680.1634589924337668No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15540.16199953714417958No Hit
GAGTATTAGTAGTTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGGTCCTGATCTATAAGGCGT15050.15689144363062438No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14500.15115786927867467No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA13290.13854400570438524No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13110.1366675631892017No Hit
CCTTCCACCCTGTCTGCATCTGCAGGAGACAGAGTCACCATCACTTGCCG12890.1343741334484218No Hit
GTATTAGTAGTTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCT12310.12832781867727483No Hit
GTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCCCTTGGCCGAACGAC12160.12676411658128853No Hit
GATCTATAAGGCGTCTAATTTACAAAATGGGGTCCCATCAAGATTCAGCGGCAGTGGATCTGGGACAGAATTCA12000.12509616767890316No Hit
GTCTGCATCTGCAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA11900.1240536996149123No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11310.11790313803736624No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11290.11769464442456805No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11120.1159224487157836No Hit
GTCCTGATCTATAAGGCGTCTAATTTACAAAATGGGGTCCCATCAAGATT11010.11477573384539365No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10430.10872941907424667No Hit
TCTATAAGGCGTCTAATTTACAAAATGGGGTCCCATCAAGATTCAGCGGCAGTGGATCTGGGACAGAATTCACT10370.10810393823585214No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10350.10789544462305398No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10070.10497653404387956No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG10050.10476804043108139No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9860.10278735110949876No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9800.10216187027110425No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCC9750.10164063623910881No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT5750.050.530761
TAGTGAT3150.040.44518770
GTATCAA55300.038.4476661
ATTCGCA952.200405E-433.5269370
TATCAAC67700.031.646462
TCTCCGA2800.030.51219711
TCAACGC70750.030.475384
ATCAACG71600.030.113593
AACGCAG72250.029.889976
CGAGACT2750.029.82895323
CAACGCA72450.029.8074595
ACGAGAC2950.028.9637522
GGTATCA38750.028.6692521
TCGTATG2450.028.24587240
ACGCAGA78300.027.5339537
CGCAGAG78550.027.4926838
TAGGCAT3000.027.39749329
GCAGAGT80300.026.8963329
ATCTCCG3050.026.89074910
CGAGCCC3050.026.88794715
Produced by FastQC (version 0.11.5)