FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757476

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757476
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences959262
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGTACTTTTTCCTGGTCGTTCGGCCAAGGGACCAAGGTGGAAGTCAAACGAACTGTGGCTGCACCAT24990.26051276919131583No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT23340.24331204613546661No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21580.22496460820922748No Hit
GTACTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC20250.21109978295814907No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15830.1650226945297531No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14770.15397253305144998No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14700.15324280540665636No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14090.14688375021631211No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC12930.13479112067401813No Hit
GAGTATTAGTAGTTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGGTCCTGATCTATAAGGCGT12570.13103823564365105No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12420.12947453354766478No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA12180.1269726101940867No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC12080.12593014213009585No Hit
GTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCCCTTGGCCGAACGAC11850.12353246558291686No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11220.11696491677977444No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10990.11456724023259549No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10370.10810393823585214No Hit
GATCTATAAGGCGTCTAATTTACAAAATGGGGTCCCATCAAGATTCAGCGGCAGTGGATCTGGGACAGAATTCA10350.10789544462305398No Hit
GTATTAGTAGTTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCT10310.10747845739745762No Hit
TCTATAAGGCGTCTAATTTACAAAATGGGGTCCCATCAAGATTCAGCGGCAGTGGATCTGGGACAGAATTCACT10020.10445530001188413No Hit
GTCCTGATCTATAAGGCGTCTAATTTACAAAATGGGGTCCCATCAAGATT9960.10382981917348962No Hit
CCTTCCACCCTGTCTGCATCTGCAGGAGACAGAGTCACCATCACTTGCCG9840.10257885749670058No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9620.1002854277559207No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT7300.048.5415341
GTATCAA61550.040.9644051
TATCAAC70750.035.476452
ATCAACG74500.033.537843
TCAACGC74250.033.5149734
AACGCAG75550.033.0370066
CAACGCA75600.032.9666755
CGCAGAG81300.030.7439278
ACGCAGA82050.030.4214027
AGAGTAC82050.030.33681511
GCAGAGT83250.030.0647039
GCTTGTA2700.027.7627161
GGTATCA35050.027.4134771
AGTACGG61100.026.134713
GAGTACG61450.026.0993812
CAGAGTA96350.025.80031210
ACGGGGA9450.025.60205816
GAGTACT36700.024.03061512
TACGGGA26150.023.31448215
GTACGGG68650.023.00887714
Produced by FastQC (version 0.11.5)