EGAF00005757477 FastQC Report

Basic Statistics

Measure	Value
Filename	EGAF00005757477
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	868419
Sequences flagged as poor quality	0
Sequence length	20-76
%GC	53

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT	6603	0.7603472517298677	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2373	0.27325519133045223	No Hit
CTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTTCC	2272	0.2616248608102771	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1675	0.19287924377518226	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATGTCGTATGCCGT	1393	0.16040643974855456	No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT	1153	0.13277001078972248	TruSeq Adapter, Index 3 (95% over 21bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1091	0.12563059997535753	No Hit
GTATCAACGCAGAGTACGGGCTAGTCCCTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGC	1015	0.11687906413839402	No Hit
GTCCCTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGC	916	0.10547903719287578	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAATTG	35	1.5413752E-7	65.98244	62
AGGGGGG	1995	0.0	62.311615	70
ATACGGG	150	0.0	56.271564	1
CTTATGG	20	0.0055803037	55.49788	51
GTATTCT	20	0.005740031	55.10348	49
AAGTACG	195	0.0	54.939693	1
CTTGTAA	35	1.32804635E-5	53.967865	57
CTTATAC	1945	0.0	52.74416	1
CGTATGA	20	0.006890666	52.6164	41
CTAGTAT	40	8.131028E-7	51.870457	39
TTATACA	2020	0.0	51.071957	2
TATACAC	2060	0.0	50.23775	3
CTATTAC	20	0.009357801	48.696545	1
TCCTACC	20	0.009383391	48.6629	2
TAACAAC	20	0.009383391	48.6629	2
GTGGTAT	1235	0.0	47.842224	1
ATATCAA	380	0.0	46.987896	1
AGTTTCC	30	7.0719863E-4	44.948467	35
ATACACA	2365	0.0	44.17041	4
CTTGAAA	1540	0.0	43.66491	57