FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757480

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757480
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences409187
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC14400.35191733852737256No Hit
GCCTTGGGCTGACTCAGGACGGTCAACTTGGTCCCGCCGCCGAACACCCAAGCACCGCTGCTGTCTGCTGATTGA14200.3470295977144924No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG12560.306950123048875No Hit
CCCAAGCACCGCTGCTGTCTGCTGATTGACAGTGATAGTCAGCCTCGTCT12180.2976634155044026No Hit
CCTCTGAGGAGCTTCAAGCCAACAGGGCCACACTGGTGTGTCTCATAAGT11300.2761573559277299No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAGGGCCACACTGGTGT11050.27004767991162965No Hit
GGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC10800.26393800389552946No Hit
GTGCTTGGGTGTTCGGCGGCGGGACCAAGTTGACCGTCCTGAGTCAGCCC10380.25367374818848104No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG10220.2497635555381769No Hit
GTGTGGCCCTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA9850.2407212350343486No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTCAGGACGGTCAACTTGGTCCCGC9530.2329008497337403No Hit
CCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATGCATTGCCGAAG9480.23167891453052028No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC8870.21677130505123574No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG8810.2153049828073717No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT8700.2126167253602876No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTCAGGACGGTCAACTTGGTCCCGCCGCCGAACAC8640.21115040311642355No Hit
CACCAGTGTGGCCCTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG8410.20552950118161137No Hit
GACTATCACTGTCAATCAGCAGACAGCAGCGGTGCTTGGGTGTTCGGCGGCGGGACCAAGTTGACCGTCCTGAGT8230.2011305344500192No Hit
GTCCAGGAGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTAC7820.19111066578361482No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC7410.18109079711721046No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC7360.17986886191399043No Hit
CTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGG7360.17986886191399043No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG7340.1793800878327024No Hit
GACTCAGGACGGTCAACTTGGTCCCGCCGCCGAACACCCAAGCACCGCTGCTGTCTGCTGATTGACAGTGATAGT7250.17718060446690634No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA7160.17498112110111025No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA7100.1735147988572462No Hit
GGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCAC7080.1730260247759582No Hit
CCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATG7020.17155970253209413No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC6970.17033776732887407No Hit
GTGATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATGGTCAACGTGA6880.168138283963078No Hit
TCCTGAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAGGG6770.16545002651599391No Hit
GTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATGC6660.16276176906890982No Hit
GTCCTGAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC6650.16251738202826582No Hit
GGCCTGGCCTGGCTTCTGCTGGTACCAATAAGCATATTGCTTCGGCAATGCATCTCCAGAGCAGGTGATCCTGGC6400.15640770601216558No Hit
CTTATGAGACACACCAGTGTGGCCCTGTTGGCTTGAAGCTCCTCAGAGGA6160.15054241703670937No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA6060.14809854663026928No Hit
TCAGTGGAGTCCAGGCAGAAGACGAGGCTGACTATCACTGTCAATCAGCA6010.14687661142704925No Hit
CCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAATAAGCATATTGCTTC5990.14638783734576125No Hit
GGCCAGGCCCCTGTGGTGTTGATATATAAAGACATTCAGAGGCCCTCAGG5980.14614345030511722No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT5950.1454102891831852No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAGGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC5830.14247764469545707No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA5760.14076693541094903No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5590.13661235572000086No Hit
CTATATACACTGGGTGCGACAGGCCCCTGGACAAGGGCTCGAGTGGATGGGATGGATCAAACCTAACAGTGGTGA5560.13587919459806885No Hit
GTATCAACGCAGAGTACGGGGTGGGTCCAGGAGGCAGAACTCTGGGTGTC5450.13319093715098476No Hit
GTGTATATAGTTGCCGATGAAGGTGTATCCAGAAGCCTTGCAGGAGACCTTCACTGAGGCCCCAGGCTTCTTCAC5370.1312358408258327No Hit
GCTCTGGAGATGCATTGCCGAAGCAATATGCTTATTGGTACCAGCAGAAG5360.13099145378518867No Hit
TCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCA5340.13050267970390064No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC5270.1287919704193926No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG5260.1285475833787486No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT5230.12781442225681658No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT5220.12757003521617255No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC5210.12732564817552855No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT5170.12634810001295252No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG5170.12634810001295252No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT5100.12463739072844444No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT5090.12439300368780044No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCCTGTTGGCTTGAAG5060.12365984256586841No Hit
GGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTCAGGACGGTCAACTTGGTCCCGCCGCCGAACACC5050.1234154555252244No Hit
CTGTAGGACAGCTGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT5040.1231710684845804No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA4960.12121597215942832No Hit
ACGTGACTGTTGTCCCTGAGCTGGAGCCAGAGAATCGCTCAGGGATCCCT4950.12097158511878432No Hit
GTAATACATGGCCGTGTCGTCAGATCTCAGGCCGCTCAACTCCATATAGACTGTGTTGATGGACGTGTCCCTGGT4920.1202384239968523No Hit
ACTCAGGACGGTCAACTTGGTCCCGCCGCCGAACACCCAAGCACCGCTGC4900.11974964991556428No Hit
CTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGT4820.1177945535904122No Hit
GTCTATATGGAGTTGAGCGGCCTGAGATCTGACGACACGGCCATGTATTACTGTGCGAGAGATGAGTCTGAGGTG4760.11632823134654816No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC4760.11632823134654816No Hit
GTGGAGTCCAGGCAGAAGACGAGGCTGACTATCACTGTCAATCAGCAGAC4740.11583945726526014No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACTCAGGACGGTCAACTTGGTCCCGCCGCCGAACACCCAAGCACCG4690.1146175220620401No Hit
GTCCAGGCAGAAGACGAGGCTGACTATCACTGTCAATCAGCAGACAGCAG4640.11339558685882006No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA4570.11168487757431199No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA4550.11119610349302397No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCATCGG4530.11070732941173594No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAGGGCC4510.11021855533044794No Hit
CTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGT4500.10997416828980393No Hit
CAGTCACGTTGACCATCAGTGGAGTCCAGGCAGAAGACGAGGCTGACTAT4500.10997416828980393No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG4490.10972978124915993No Hit
CTCCACTGATGGTCAACGTGACTGTTGTCCCTGAGCTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGCCT4390.10728591084271982No Hit
CCACTGATGGTCAACGTGACTGTTGTCCCTGAGCTGGAGCCAGAGAATCG4360.1065527497207878No Hit
GGCCTGGCTTCTGCTGGTACCAATAAGCATATTGCTTCGGCAATGCATCTCCAGAGCAGGTGATCCTGGCCGT4360.1065527497207878No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA4340.10606397563949978No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCACGCGACCCCGAGAGCCT4340.10606397563949978No Hit
CCGCTGCTGTCTGCTGATTGACAGTGATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATGGTCAACGTGA4230.10337571819241569No Hit
CCCCTGTGGTGTTGATATATAAAGACATTCAGAGGCCCTCAGGGATCCCT4230.10337571819241569No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACA150.002385319268.77376620
TGGTATC950.057.8935052
GTGGTAT1050.052.392651
GCCATAG200.00705259852.30347455
GCCTTAA200.00734064651.77671437
GCTAGAC200.007455395551.57401319
GGTATCA2800.041.7503931
GGGTGCA451.0207032E-438.20297619
GTATCAA6700.036.9485471
GCTTGTC709.978139E-734.3826751
TTCGGGG2700.033.5525670
ATCAACG7400.033.4166223
TCAACGC7550.032.7567184
CAACGCA7600.032.549175
AACGCAG7750.031.9230886
CGCAGAG8000.031.3550158
GCAGAGT8300.030.6356959
AGAGTAC8150.030.3563111
ACGGGGA1501.8189894E-1229.79467416
CGGGGGA703.9354243E-529.47086517
Produced by FastQC (version 0.11.5)