FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005757481

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757481
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1040862
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT30690.29485176709304406No Hit
GTTATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATTTTTTTGGGTT30600.2939870991543548No Hit
GTCTACAAACTCCGTTCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA25670.24662251095726426No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24430.23470930824643418No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21010.20185192657624162No Hit
ATCCATAACTGTGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCC20950.2012754812837821No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20740.1992579227601738No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19990.1920523566044298No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19830.19051516915787106No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19510.18744079426475366No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19150.18398212250999654No Hit
CTCCCTGACCGTCGCCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTA19000.18254100927884773No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA18120.17408647832277477No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17970.17264536509162598No Hit
GTTTGTAGACCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTACTGATTTTCAGTGTAAAAT17710.17014743549096806No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG17330.16649661530539112No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTACTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA16710.16054001394997608No Hit
GGTCTAGTCAGAGCCTCCTGCACAGTTATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTC16480.15833030699554793No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG16420.1577538617030884No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT16400.15756171327226856No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16120.1548716352407908No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAACCCAAAAAAATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTA16030.15400696730210153No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15620.15006792447029482No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG15340.14737784643881705No Hit
GAGTTTGTAGACCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTACTGATTTTCAGTGTAAAAT14890.14305450674537065No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14330.13767435068241515No Hit
CTACAAACTCCGTTCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACG13850.13306278834273902No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13770.13229419461945965No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGTAGAG13480.12950804237257196No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12920.12412788630961646No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12830.12326321837092717No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA12600.12105351141649902No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC12540.1204770661240395No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12530.12038099190862957No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12510.12018884347780974No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATTTTTTTGGGTTCTAATCGGGCC12440.1195163239699403No Hit
GTCTCCACTCTCCCTGACCGTCGCCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTGCA12340.11855558181584111No Hit
CTCCTGCACAGTTATGGATACAACTATTTGGATTGGTACCTGCAGAAGCC12160.11682624593846254No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12100.11624980064600303No Hit
TTATTACTGCATGCAAGGTCTACAAACTCCGTTCACTTTTGGCCAGGGGA12040.1156733553535435No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA11730.112695054675836No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACTCTACTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA11690.1123107578141963No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11510.11058142193681776No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11170.10731489861288049No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11090.10654630488960111No Hit
GGCCAAAAGTGAACGGAGTTTGTAGACCTTGCATGCAGTAATAAACCCCA10990.10558556273550192No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10590.10174259411910513No Hit
GCCCTGGCTTCTGCAGGTACCAATCCAAATAGTTGTATCCATAACTGTGC10420.10010933245713648No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)