FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757482

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757482
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1040862
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT29140.2799602637045065No Hit
GTTATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATTTTTTTGGGTT29110.2796720410582767No Hit
GTCTACAAACTCCGTTCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA24960.23980124166315997No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20070.19282095032770913No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19840.19061124337328098No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18860.18119597026310885No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG18660.17927448595491044No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18570.1784098180162212No Hit
ATCCATAACTGTGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCC18180.17466292361523428No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17800.17101210342965734No Hit
GTTTGTAGACCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTACTGATTTTCAGTGTAAAAT17710.17014743549096806No Hit
CTCCCTGACCGTCGCCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTA17070.1639986857047332No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16830.16169290453489513No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA16600.15948319758046697No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTACTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA16450.15804208434931816No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16320.15679311954898922No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15790.15170118613226344No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT15530.14920325653160554No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14860.1427662840991409No Hit
GAGTTTGTAGACCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTACTGATTTTCAGTGTAAAAT14660.1408447997909425No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAACCCAAAAAAATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTA14300.13738612803618538No Hit
GGTCTAGTCAGAGCCTCCTGCACAGTTATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTC14300.13738612803618538No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG14230.13671360852831596No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14040.13488819843552746No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13550.13018056188044141No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13290.1276826322797835No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12970.12460825738666605No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGTAGAG12780.12278284729387759No Hit
CTACAAACTCCGTTCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACG12770.12268677307846765No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC12730.12230247621682798No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG12500.12009276926239981No Hit
CTCCTGCACAGTTATGGATACAACTATTTGGATTGGTACCTGCAGAAGCC12500.12009276926239981No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12380.11893987867748078No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACTCTACTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA11800.11336757418370542No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA11790.11327149996829551No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11650.11192646095255664No Hit
GGCCAAAAGTGAACGGAGTTTGTAGACCTTGCATGCAGTAATAAACCCCA11270.10827564076697968No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11200.10760312125911023No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11080.1064502306741912No Hit
TTATTACTGCATGCAAGGTCTACAAACTCCGTTCACTTTTGGCCAGGGGA10830.1040483752889432No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA10700.10279941048861425No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10680.10260726205779441No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT10610.10193474254992496No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10560.10145437147287538No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10490.10078185196500593No Hit
GTCTCCACTCTCCCTGACCGTCGCCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTGCA10410.10001325824172656No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4250.044.4526141
TGGTATC4200.041.688362
GGTATCA10700.039.165131
TTAACGG459.9995385E-538.3433735
GTATCAA23550.038.0691451
TATCAAC26300.034.070512
ATCAACG27550.032.141553
TCAACGC28250.031.2236274
CAACGCA28250.031.1066175
AACGCAG29400.030.127726
ACGCAGA30200.029.3282247
AGAGTAC31600.028.02887311
CGCAGAG31650.027.9845948
GCAGAGT32300.027.315159
CAGAGTA32600.027.06378410
GTATATA907.1434406E-626.7164671
TACGGGT3700.025.06241615
CGGTACC700.001286631624.79543145
TTTAACG700.001333224524.64454734
CGCGGTA851.3926532E-424.48112143
Produced by FastQC (version 0.11.5)