FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757483

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757483
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences563517
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT72231.2817714461143142No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATGTCGTATGCCGT15260.2707992837838078No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT13030.23122638713650162TruSeq Adapter, Index 3 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8820.15651701723284303No Hit
CTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTTCC8620.15296787852008012No Hit
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCT7530.13362507253552244RNA PCR Primer, Index 3 (95% over 23bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6530.11587937897170804No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG20100.068.4413370
TAATAAG200.00572342155.14108753
TCCGTGT200.006270401653.88362546
CTTATAC21150.053.1465641
TTAGGGT400.00715473952.1087470
ATTCCGT551.9826984E-951.72376644
TTATACA22300.050.5307732
TATACAC22800.050.143093
CTTGTAT800.049.3048116
CTCCTAC200.00891141149.300431
TCCTACA200.00892705649.2785572
TTGAGTA401.1572629E-649.2785573
CGTATGG1000.048.9980841
ATTCTGC451.2526107E-648.7143751
TGTTATG304.822517E-448.57533650
CATATAC353.0253674E-546.9527931
TTGTATC850.046.4086517
AATGTCG4050.044.60716236
AAGGGGG20150.043.46786569
GTATGGC1150.042.86232842