FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757485

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757485
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences806485
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT54970.6815997817690348No Hit
GTCTGGGACAGACTTCTCTCTCACCATCAGCAGTCTGCAACCTGAAGATT43030.5335499110336832No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC39320.48754781552043747No Hit
GAGTTACAGTTCCCCGTGGACGTTCGGCCAAGGGACCAAGGTGGCTATCAAACGAACTGTGGCTGCACCAT38610.4787441799909484No Hit
GATCTATGCTGCGTCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCT37230.4616328883984203No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC34990.4338580382772153No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT33370.4137708698859867No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32920.40819110088842325No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG31640.3923197579620204No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC31250.387483958164132No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29960.37148862037111663No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG29860.37024867170499143No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT27640.3427218113170115No Hit
GTGCAGCCACAGTTCGTTTGATAGCCACCTTGGTCCCTTGGCCGAACGTC27500.3409858831844362No Hit
GTCCCAGACCCACTGCCACTGAACCTTGATGGGACCCCACTTTGCAAACT26350.3267264735239961No Hit
GGTCAGGGCATCAGCTACTATTTAAATTGGTATCAGCAGCAGCCAGGGAAAGCCCCCCAGCTCCTGATCTATG26300.3261064991909335No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGAGAAGTCTGTC26150.3242465761917457No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24320.3015555156016541No Hit
ACTTACTACTGTCAACAGAGTTACAGTTCCCCGTGGACGTTCGGCCAAGGGACCAAGGTGGCTATCAAACGAA23760.2946118030713528No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23690.2937438390050652No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG22600.28022839854430026No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22590.28010440367768774No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG22090.2739046603470616No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21860.27105277841497366No Hit
GCATAGATCAGGAGCTGGGGGGCTTTCCCTGGCTGCTGCTGATACCAATTTAAATAGTAGCTGATGCCCTGAC21800.2703088092152985No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21030.26076120448613427No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATAGCCACCTTGGTCCCTTGGCCGAACGTCCACGGGGAA21000.2603892198862967No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCAGGTCAGGGCATCAGCTA20780.2576613328208212No Hit
CCTTGGTCCCTTGGCCGAACGTCCACGGGGAACTGTAACTCTGTTGACAG20700.25666937388792105No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCAGGTCAGG20210.25059362542390745No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20170.25009764595745737No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT19860.2462538050924692No Hit
GGACCAAGGTGGCTATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC19660.24377390776021873No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18730.23224238516525417No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT18680.23162241083219157No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC18460.22889452376671604No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18110.22455470343527775No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGAGAAGTCTGTCCCAGACCCACTGCCA17970.22281877530270247No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17950.2225707855694774No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17520.21723900630513898No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17160.21277519110708817No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16960.21029529377483772No Hit
CCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAG16870.20917933997532503No Hit
GTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTTCCCCGTGGACGTTCGGCCAAGGGA16750.20769140157597477No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16670.20669944264307458No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA16660.20657544777646203No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC16640.20632745804323702No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16630.20620346317662447No Hit
CTCCTGATCTATGCTGCGTCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGGTCTGGGACAGA16290.20198763771179873No Hit
GACTTCTCTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTTCC16140.20012771471261093No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA16110.19975573011277334No Hit
GTGGGAGACAGAGTCACCATCACTTGCCGGGCAGGTCAGGGCATCAGCTACTATTTAAATTGGTATCAGCAGCAG16040.1988877660464857No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGACTTCTCTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTA15350.19033212025022164No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15230.18884418185087137No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT14870.18438036665282057No Hit
GATCAGGAGCTGGGGGGCTTTCCCTGGCTGCTGCTGATACCAATTTAAAT14540.18028853605460735No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14170.17570072598994402No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC14030.1739647978573687No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA14020.1738408029907562No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13770.17074093132544313No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC13640.16912899805948034No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13590.16850902372641774No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA13310.1650371674612671No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC13090.16230928039579162No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC13050.16181330092934154No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG12890.15982938306354116No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCTCT12730.15784546519774081No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC12680.1572254908646782No Hit
GAACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGAGAAGT12630.15660551653161559No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT12600.15623353193177802No Hit
CATCAAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCTCTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTG12590.1561095370651655No Hit
TCTATGCTGCGTCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCTCT12560.15573755246532794No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC12440.15424961406597767No Hit
GCCAAGGGACCAAGGTGGCTATCAAACGAACTGTGGCTGCACCATCTGTC12210.15139773213388966No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT12120.15028177833437698No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC12060.14953780913470183No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11940.14804987073535156No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA11840.14680992206922633No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG11810.14643793746938877No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC11760.14581796313632617No Hit
GGGACAGACTTCTCTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGC11740.1455699734031011No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11450.14197412227133796No Hit
ATGCTGCGTCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCTCTCTCA11430.14172613253811292No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC11410.14147814280488788No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT11410.14147814280488788No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG11390.14123015307166284No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT11380.14110615820505032No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT11280.1398662095389251No Hit
GGGCATCAGCTACTATTTAAATTGGTATCAGCAGCAGCCAGGGAAAGCCCCCCAGCTCCTGATCTATGCTGCGTC11280.1398662095389251No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC11030.13676633787361203No Hit
GTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCA10950.13577437894071184No Hit
ACTGTCAACAGAGTTACAGTTCCCCGTGGACGTTCGGCCAAGGGACCAAGGTGGCTATCAAACGAACTGTGGCTG10840.1344104354079741No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA10820.13416244567474905No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC10810.13403845080813653No Hit
GCCACAGTTCGTTTGATAGCCACCTTGGTCCCTTGGCCGAACGTCCACGG10680.13242651754217374No Hit
GTTACAGTTCCCCGTGGACGTTCGGCCAAGGGACCAAGGTGGCTATCAAA10390.1288306664104106No Hit
CCATCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCG10310.12783870747751042No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC10200.1264747639447727No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT10050.12461484094558484No Hit
GCCAGGGAAAGCCCCCCAGCTCCTGATCTATGCTGCGTCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGG10020.12424285634574728No Hit
ACCTTGATGGGACCCCACTTTGCAAACTGGACGCAGCATAGATCAGGAGCTGGGGGGCTTTCCCTGGCTGCTG10000.12399486661252225No Hit
CTGCTGATGGTGAGAGAGAAGTCTGTCCCAGACCCACTGCCACTGAACCT9810.12163896414688431No Hit
GCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATAGCCACCTTGGTCCCTTGGC9490.1176711284152836No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT9490.1176711284152836No Hit
CTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTG9410.11667916948238342No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC9390.11643117974915837No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG9310.1154392208162582No Hit
GTATCAACGCAGAGTACGGGAGTCAGTCTCAGTCAGGACACAGCATGGAC9280.11506723621642063No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA9280.11506723621642063No Hit
GGTATCAGCAGCAGCCAGGGAAAGCCCCCCAGCTCCTGATCTATGCTGCGTCCAGTTTGCAAAGTGGGGTCCCAT9020.11184336968449507No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA8980.11134739021804498No Hit
ACCCAGTCTCCATCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCAT8970.11122339535143246No Hit
CTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAAT8940.11085141075159487No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT8850.10973545695208219No Hit
CACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTTCCCCGTGGACGTT8780.10886749288579452No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA8770.10874349801918201No Hit
GATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGG8680.10762754421966932No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG8670.10750354935305678No Hit
GAGTCACCATCACTTGCCGGGCAGGTCAGGGCATCAGCTACTATTTAAATTGGTATCAGCAGCAGCCAGGGAAAG8640.10713156475321921No Hit
GTCCCTTGGCCGAACGTCCACGGGGAACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGA8590.1065115904201566No Hit
GCTCCTGATCTATGCTGCGTCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGGTCTGGGACAGA8580.10638759555354409No Hit
GCCCTGACCTGCCCGGCAAGTGATGGTGACTCTGTCTCCCACAGATGCAGACAGGGAGGATGGAGACTGGGTCAT8360.1036597084880686No Hit
GGGAAAGCCCCCCAGCTCCTGATCTATGCTGCGTCCAGTTTGCAAAGTGG8330.10328772388823101No Hit
GTGGCAGTGGGTCTGGGACAGACTTCTCTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTA8280.10266774955516843No Hit
GTATCAACGCAGAGTACGGGGAGTCAGTCTCAGTCAGGACACAGCATGGA8250.10229576495533084No Hit
TTACTACTGTCAACAGAGTTACAGTTCCCCGTGGACGTTCGGCCAAGGGA8190.1015517957556557No Hit
GGTTGCAGACTGCTGATGGTGAGAGAGAAGTCTGTCCCAGACCCACTGCC8130.10080782655598058No Hit
CCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAAC8070.10006385735630546No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTATG554.0017767E-1156.5539240
GTGGTAT2050.055.2166941
ACCGACG200.00685733652.68085560
CTTATAC2800.046.551731
TATACAC3100.045.34373
GAGCGAT2750.042.5302332
CGTATGC2850.042.4801841
GTATCAA17350.041.3196951
ATGCCGT2900.039.4213744
CAATTAG350.001386391839.2014581
AGCGATC1700.038.45841233
ATCTCCG3500.038.19772310
TCGTATG2450.038.08733740
TATGCCG3100.037.97653643
TGCCGTC2750.037.81371345
GTATGCC3300.037.7498142
ATACACA3700.037.0640644
TACACAT3650.036.6324965
ACGAGAC3700.036.15091722
TCTCCGA3700.036.13522311
Produced by FastQC (version 0.11.5)