FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005757486

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757486
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences806485
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT49510.6138985845985976No Hit
GTCTGGGACAGACTTCTCTCTCACCATCAGCAGTCTGCAACCTGAAGATT38950.4829600054557741No Hit
GAGTTACAGTTCCCCGTGGACGTTCGGCCAAGGGACCAAGGTGGCTATCAAACGAACTGTGGCTGCACCAT35790.44377762760621714No Hit
GATCTATGCTGCGTCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCT34920.4329900742109277No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC32620.40447125489004754No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT32010.39690756802668364No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31110.3857480300315567No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29860.37024867170499143No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29480.36553686677371555No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28700.35586526717793887No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG27530.34135786778427374No Hit
GTGCAGCCACAGTTCGTTTGATAGCCACCTTGGTCCCTTGGCCGAACGTC26380.3270984581238337No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26010.32251064805917035No Hit
GTCCCAGACCCACTGCCACTGAACCTTGATGGGACCCCACTTTGCAAACT25710.3187908020607947No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24810.3076312640656677No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC24340.3018035053348791No Hit
GGTCAGGGCATCAGCTACTATTTAAATTGGTATCAGCAGCAGCCAGGGAAAGCCCCCCAGCTCCTGATCTATG24260.30081154640197894No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGAGAAGTCTGTC23150.287048116207989No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21760.2698128297488484No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21600.267828911883048No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21160.26237313775209703No Hit
ACTTACTACTGTCAACAGAGTTACAGTTCCCCGTGGACGTTCGGCCAAGGGACCAAGGTGGCTATCAAACGAA20580.25518143548857075No Hit
GCATAGATCAGGAGCTGGGGGGCTTTCCCTGGCTGCTGCTGATACCAATTTAAATAGTAGCTGATGCCCTGAC20450.253569502222608No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20160.24997365109084485No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCAGGTCAGG19560.2425339590940935No Hit
CCTTGGTCCCTTGGCCGAACGTCCACGGGGAACTGTAACTCTGTTGACAG19180.23782215416281766No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG19100.2368301952299175No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATAGCCACCTTGGTCCCTTGGCCGAACGTCCACGGGGAA19030.23596223116362983No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18860.23385431843121696No Hit
GGACCAAGGTGGCTATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC18290.2267866110343032No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18270.22653862130107813No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18120.2246786983018903No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCAGGTCAGGGCATCAGCTA18000.22319075990254006No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17950.2225707855694774No Hit
GTGGGAGACAGAGTCACCATCACTTGCCGGGCAGGTCAGGGCATCAGCTACTATTTAAATTGGTATCAGCAGCAG17910.22207480610302735No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT17860.22145483176996472No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17620.2184789549712642No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC17290.21438712437305096No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17230.21364315517337584No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16960.21029529377483772No Hit
CTCCTGATCTATGCTGCGTCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGGTCTGGGACAGA16700.20707142724291214No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16230.2012436685121236No Hit
GACTTCTCTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTTCC16160.20037570444583594No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT16130.20000371984599838No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT16030.19876377117987315No Hit
GTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTTCCCCGTGGACGTTCGGCCAAGGGA16000.1983917865800356No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCTCT15800.19591188924778513No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGAGAAGTCTGTCCCAGACCCACTGCCA15420.19120008431650928No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15380.1907041048500592No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15060.1867362691184585No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14810.18363639745314544No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14600.18103250525428247No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14520.18004054632138228No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGACTTCTCTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTA14510.17991655145476979No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC13880.17210487485818088No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13510.16751706479351755No Hit
GATCAGGAGCTGGGGGGCTTTCCCTGGCTGCTGCTGATACCAATTTAAAT13280.16466518286142953No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC13170.16330123932869178No Hit
CCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAG13140.16292925472885422No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC13070.16206129066256655No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12690.15734948573129073No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12550.15561355759871542No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12400.15375363459952757No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA11950.14817386560196408No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC11940.14804987073535156No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC11730.1454459785364886No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC11550.1432140709374632No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11490.14247010173778807No Hit
CATCAAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCTCTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTG11440.14185012740472544No Hit
TCTATGCTGCGTCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCTCT11410.14147814280488788No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT11370.14098216333843777No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA11180.13862626087279986No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC11180.13862626087279986No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11160.13837827113957482No Hit
GGGACAGACTTCTCTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGC11150.1382542762729623No Hit
GAACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGAGAAGT11100.1376343019398997No Hit
GCCCTGACCTGCCCGGCAAGTGATGGTGACTCTGTCTCCCACAGATGCAGACAGGGAGGATGGAGACTGGGTCAT11000.13639435327377447No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10980.13614636354054943No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC10970.13602236867393688No Hit
GGGCATCAGCTACTATTTAAATTGGTATCAGCAGCAGCCAGGGAAAGCCCCCCAGCTCCTGATCTATGCTGCGTC10950.13577437894071184No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC10840.1344104354079741No Hit
ATGCTGCGTCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCTCTCTCA10810.13403845080813653No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA10640.13193053807572366No Hit
CTGCTGATGGTGAGAGAGAAGTCTGTCCCAGACCCACTGCCACTGAACCT10620.13168254834249862No Hit
ACTGTCAACAGAGTTACAGTTCCCCGTGGACGTTCGGCCAAGGGACCAAGGTGGCTATCAAACGAACTGTGGCTG10440.1294506407434732No Hit
GCCAAGGGACCAAGGTGGCTATCAAACGAACTGTGGCTGCACCATCTGTC10350.12833468694396052No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT10230.12684674854461025No Hit
GTTACAGTTCCCCGTGGACGTTCGGCCAAGGGACCAAGGTGGCTATCAAA10070.12486283067880989No Hit
GTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCA9880.12250692821317198No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT9860.12225893847994693No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC9830.12188695388010937No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA9610.11915906681463388No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC9580.11878708221479631No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC9580.11878708221479631No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC9510.11791911814850865No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT9510.11791911814850865No Hit
GCCACAGTTCGTTTGATAGCCACCTTGGTCCCTTGGCCGAACGTCCACGG9510.11791911814850865No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT9490.1176711284152836No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT9440.11705115408222098No Hit
ACCTTGATGGGACCCCACTTTGCAAACTGGACGCAGCATAGATCAGGAGCTGGGGGGCTTTCCCTGGCTGCTG9270.11494324134980811No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT9210.114199272150133No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG9150.11345530295045785No Hit
GTATCAACGCAGAGTACGGGAGTCAGTCTCAGTCAGGACACAGCATGGAC9080.1125873388841702No Hit
CCATCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCG9020.11184336968449507No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG8980.11134739021804498No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC8950.11097540561820742No Hit
GGTATCAGCAGCAGCCAGGGAAAGCCCCCCAGCTCCTGATCTATGCTGCGTCCAGTTTGCAAAGTGGGGTCCCAT8750.10849550828595696No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA8660.10737955448644426No Hit
GGTTGCAGACTGCTGATGGTGAGAGAGAAGTCTGTCCCAGACCCACTGCC8610.10675958015338165No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC8550.10601561095370653No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG8500.10539563662064391No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT8440.10465166742096878No Hit
GCTCCTGATCTATGCTGCGTCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGGTCTGGGACAGA8440.10465166742096878No Hit
GCCAGGGAAAGCCCCCCAGCTCCTGATCTATGCTGCGTCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGG8380.10390769822129363No Hit
GCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATAGCCACCTTGGTCCCTTGGC8210.10179978548888076No Hit
CACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTTCCCCGTGGACGTT8180.1014278008890432No Hit
CTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAAT8140.1009318214225931No Hit
GTGGCAGTGGGTCTGGGACAGACTTCTCTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTA8090.10031184708953049No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG8090.10031184708953049No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)