FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005757494

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757494
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences176780
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17731.0029415092205No Hit
CAATTAAACTATGCAAAAGATTCGCTCGGGGCAAAAATTCGCGCGATTCT6650.3761737753139495No Hit
CTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTTCC5800.32809141305577555No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3140.17762190293019572No Hit
ATTCTTAGGTAGAATCCATAACCCTTCTTATAAAAGTTTACATTCCGTTGCCTAACTTTTATAAGGTTGCTAGTT3030.17139947957913793No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2670.15103518497567597No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2620.14820681072519515No Hit
GTATCAACGCAGAGTACGGGCTAGTCCCTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGC2490.14085303767394503No Hit
GTATCAACGCAGAGTACGGGTTCTAATACGACTCACTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGG2270.12840819097182937Clontech Universal Primer Mix Long (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2240.1267111664215409No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2120.11992306822038692No Hit
ATGCAAAAGATTCGCTCGGGGCAAAAATTCGCGCGATTCTTAGGTAGAATCCATAACCCTTCTTATAAAAGTTTA2000.11313497001923295No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1970.11143794546894445No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1890.10691254666817514Clontech SMART CDS Primer II A (100% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGG1830.10351849756759814No Hit
CTGAAAGACAGGCTATTTTATTTCAAAAAAAGAAAAAAAAGTGGGCTCTGGGAACAGGGACTAGCCCGTACTCTG1800.10182147301730966No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1770.10012444846702116No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)