FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757495

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757495
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2411389
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT67830.2812901609819071No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC17300.041.9317051
TCGTATG17650.039.99920340
GTGGTAT13300.039.6907271
CTCGTAT14900.039.1863239
AGGGGGG19600.038.0147770
CGTATGC18850.037.5256141
GTATCAA85400.037.088061
TATGCCG19950.035.56922543
ACGAGAC20150.035.49312622
GTATGCC20050.035.34015342
TCTCGTA14950.035.06390438
TATACAC21350.034.7627833
GGTATCA41900.034.3010561
CACGAGA20950.034.29393821
GACTAGG20750.034.1768526
TTATACA21500.034.04522
GCCGTCT19850.033.98064446
ATCTCCG21300.033.88325510
ATGCCGT20900.033.3304944
CCCACGA21600.033.25849519
Produced by FastQC (version 0.11.5)