FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757497

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757497
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences918184
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT29510.3213952758924137No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC7200.044.2700921
CTCGTAT6200.041.7239139
TCTCGTA5850.040.0153538
AGGGGGG8000.039.87018670
TCGTATG7350.039.02127540
GGTATCA26850.038.5186161
TATCTCG6400.038.06593336
GTATCAA53450.037.5568431
ACGGACT8000.036.9162927
GCCGTCT7950.036.57188846
CGGACTC8150.036.2586128
GACGGAC8300.035.96740326
TCCTATC6900.035.6573333
AGACGGA8350.035.33226825
TATACAC9350.035.155343
CGTATGC8200.035.07964741
TTATACA9250.034.8027082
CCGTCTT8400.034.68689347
ATCTCGT7300.033.8971337
TACCGGT502.06989E-433.8813137
Produced by FastQC (version 0.11.5)