FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757499

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757499
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences562165
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT66561.183994023107095No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT11020.19602785659014704TruSeq Adapter, Index 1 (95% over 21bp)
CTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTTCC9390.167032810651677No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9340.16614339206460738No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7680.13661469497389556No Hit
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT6900.12273976501560929RNA PCR Primer, Index 1 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATGTCGTATGCCGT6050.10761964903542555No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACAT204.6068575E-4104.03960470
GTATTCT150.001937148272.481649
GTATGAC207.7689416E-570.2810242
CGTATGA208.0054364E-569.8568441
TGAGTAC201.0696058E-465.8865054
TTGAGTA201.0696058E-465.8865053
AATTATG350.004246770459.45120270
GCTCACA301.784569E-459.40590769
AGGGGGG12350.058.408270
GAACAAT200.005783327454.9961953
GATTGAT200.006137737554.1756748
GCGTATG407.868821E-752.1125140
GTTAACT200.007442140551.5994738
CTTATAC12150.050.7118071
CACTCTA200.00809368850.51568233
CTAGCGC200.00821656250.32350532
CATAAGG200.008472485549.9345229
CTACAAT452.2115495E-549.50492569
GTAACAA200.00882391949.423671
CGCGTAT352.2454931E-549.3808439