FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757500

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757500
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences562165
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG145162.5821600419805573No Hit
CTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTTCC11460.2038547401563598No Hit
GTATCAACGCAGAGTACGGGCTAGTCCCTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGC6640.11811478836284721No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGCGT100.0077754078140.5787570
TCGAATG100.0077754078140.5787570
GTGGTAT7600.045.220441
CCGCTAT952.3705834E-644.3932970
TCTGTTA400.001204800340.33334768
CAAATAG350.001670590137.7396474
CCGCTTA400.001751464337.37727762
TATGACT451.2951173E-436.6913224
GTATCAA58550.035.3879741
GGTATCA30200.034.5776251
ATGCTTT850.003187140233.07735470
GTTAGGT850.003187140233.07735470
CGGGTAT801.0301483E-733.06041717
GATTCAG603.6002218E-430.8280659
ATAAACG752.3461544E-630.8207113
TATCAAC67450.030.7538492
ATAAGCT702.9443014E-530.75281548
ATTTGAT603.8868273E-430.4278554
TAGGGCC554.207013E-430.0201744
GAGTAAG450.005721112229.35566912
Produced by FastQC (version 0.11.5)