FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757501

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757501
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100756
Sequences flagged as poor quality0
Sequence length20-76
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT8430.8366747389733614No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5990.5945055381317242No Hit
CTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTTCC5390.5349557346460756No Hit
GTATCAACGCAGAGTACGGGCTAGTCCCTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGC4010.3979911866290841No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3670.36424629798721664No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATGTCGTATGCCGT2900.2878240501806344No Hit
CTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGCCTGT2370.23522172376831155No Hit
GTCCCTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGC2260.22430425979594265No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2070.2054468220254873No Hit
GTATCAACGCAGAGTACGGGTTCTAATACGACTCACTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGG1860.18460439080551033No Hit
GGTATCAACGCAGAGTACGGGCTAGTCCCTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGC1660.16475445631029417No Hit
TAATACGACTCACTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTT1650.16376195958553336No Hit
CTGAAAGACAGGCTATTTTATTTCAAAAAAAGAAAAAAAAGTGGGCTCTGGGAACAGGGACTAGCCCGTACTCTG1620.16078446941125094No Hit
ACTCACTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCA1480.14688951526459965No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGG1410.13994203819127396No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGG1410.13994203819127396No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1400.13894954146651317No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1390.13795704474175235No Hit
TCCCTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGCC1380.13696454801699154No Hit
CCCTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGCCT1360.13497955456746993No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA1360.13497955456746993No Hit
TTCTAATACGACTCACTATAGGGAAGTGATACCAGCATCGTCTTGATGCC1340.1329945611179483No Hit
TCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGCCTGTCTTT1330.13200206439318748No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1290.12803207749414428No Hit
ATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTTCCCC1270.12604708404462267No Hit
GTTCTAATACGACTCACTATAGGGAAGTGATACCAGCATCGTCTTGATGC1150.11413712334749294No Hit
ATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT1140.11314462662273214No Hit
TCCCTATAGTGAGTCGTATTAGAACCCGTACTCTGCGTTGATAC1070.1061971495494065No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1060.10520465282464568No Hit
CACTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTT1030.10222716265036326No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1020.10123466592560244No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1020.10123466592560244No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGG1010.10024216920084164No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCCG100.0058893254154.1090770
CCACGGT100.0058893254154.1090770
TTGTGCT204.524269E-578.30199452
CCCAGCT200.001887340272.886268
CTTGTGC304.5009256E-664.6877651
AAGTACG255.561822E-662.4394261
GGAGCGA150.003497611362.4084366
TCGGGGT200.003707504861.48288360
AGGGGGG2900.061.11221370
CGAAAGG200.00419967859.5797555
ATGTGGC200.00460237358.21898351
AGCAGAA200.004750462757.7555150
GTCTTCG200.004973247657.0912549
AGAAGCA200.00540389555.90678447
ATGCTTT402.6084308E-854.607386
ATATGCT402.6084308E-854.607384
ATATCAA700.053.5195051
GTTTGCC200.00675776952.8373942
TGTGCTT303.3121486E-452.36435753
CGTTTGC200.00715580452.0788241
Produced by FastQC (version 0.11.5)