FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757502

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757502
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100756
Sequences flagged as poor quality0
Sequence length20-76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31933.169042042161261No Hit
CTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTTCC6190.6143554726269403No Hit
GTATCAACGCAGAGTACGGGCTAGTCCCTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGC4500.44662352614236367No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2380.23621422049307236No Hit
GTATCAACGCAGAGTACGGGTTCTAATACGACTCACTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGG2350.23323673031878994No Hit
CTGAAAGACAGGCTATTTTATTTCAAAAAAAGAAAAAAAAGTGGGCTCTGGGAACAGGGACTAGCCCGTACTCTG2260.22430425979594265No Hit
GTATCAACGCAGAGTACGGGGATCTAAAAAAAAAAAAAAAAAAAAAAAAA2260.22430425979594265No Hit
GTCCCTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGC2170.21537178927309542No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2080.20643931875024812No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2030.20147683512644407No Hit
GGTATCAACGCAGAGTACGGGCTAGTCCCTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGC1980.19651435150264004No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGG1820.1806344039064671No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1730.17170193338361983No Hit
GTTCTAATACGACTCACTATAGGGAAGTGATACCAGCATCGTCTTGATGC1680.1667394497598158No Hit
TTCTAATACGACTCACTATAGGGAAGTGATACCAGCATCGTCTTGATGCC1570.15582198578744688No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1560.1548294890626861No Hit
TAATACGACTCACTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTT1550.15383699233792528No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1530.15185199888840367No Hit
ATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTTCCCC1500.14887450871412125No Hit
ACTCACTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCA1500.14887450871412125No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1480.14688951526459965No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG1420.14093453491603478No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGG1350.13398705784270912No Hit
GAGTACGGGCTAGTCCCTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGCCTGTCTTTCAGAT1200.119099606971297No Hit
ATCAACGCAGAGTACGGGCTAGTCCCTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGC1190.11810711024653618No Hit
CACTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTT1170.11612211679701456No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1160.11512962007225376No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1160.11512962007225376No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGG1100.10917463972368892No Hit
GTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGCCTGTCT1070.1061971495494065No Hit
GGTATCAACGCAGAGTACGGGTTCTAATACGACTCACTATAGGGAAGTGA1060.10520465282464568No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGTAC150.003463768962.561793
AGTTCGG200.00462513258.1466853
GAGTTCG200.00470453357.89742752
TTTATGG200.00604908354.33706745
GTGGTAT2200.054.1111951
CCGAAAT300.00919537448.8612468
AGATCTG602.5102054E-1046.921345
TTAGATC655.493348E-1043.3120083
AGGGTAT300.001012940441.74932
GTATCAA19850.038.981871
CAGGGTA350.002155297235.8028951
CAGGAGT350.002155297235.8028951
TAGATCT903.43789E-1034.7565464
ACGGGGA1101.8189894E-1234.12460716
CGGGGAT759.328869E-833.38285417
TATCAAC23250.033.2647632
TTTAGAT857.506969E-933.1538542
TTTTATG552.6756545E-432.39169344
GTGAAAG500.00415967431.30030854
GGGGATC801.6439299E-731.29642718
Produced by FastQC (version 0.11.5)