FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757503

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757503
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences355943
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGT16410.4610288726003883No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7850.22054092930609676No Hit
CTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTTCC7170.20143674689486799No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5760.16182366277746718No Hit
GTATCAACGCAGAGTACGGGCTAGTCCCTGTTCCCAGAGCCCACTTTTTTTTCTTTTTTTGAAATAAAATAGC3750.10535394712074687No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3750.10535394712074687No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTAT150.002348809569.0392439
TGACTCA150.002739182766.4136529
GATTCTC150.002834465.843723
ACAGTAT150.002843896965.788161
AGGGGGG5700.057.44399370
CTCTTAT501.6971171E-952.6305351
ATATCAA700.051.69071
CTTATAC5200.051.238861
TATACAC5400.049.929223
AATCGCG1100.049.50203736
AGGCTAG200.00887973549.341121
CTAGACT200.00889454449.3203244
TTATACA5500.048.423592
GTGGTAT4150.047.5577051
ATCTTTC353.0083998E-546.9915471
TACCACT608.565621E-943.834138
CTCCTAG307.996499E-443.8341312
ATACACA6350.041.9416924
AGTGTTG458.7439446E-539.21711343
TCTTCTG3450.039.0425750
Produced by FastQC (version 0.11.5)