FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757511

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757511
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences832314
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10360.1244722544616575No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG8250.058.79397270
GTGGTAT6850.049.8828541
CGCGTAT1800.049.8627939
ATAGCGT352.3283561E-549.08302737
TATACAC10550.048.7194943
CTTATAC10700.048.6919751
TTATACA10950.047.2486042
TAGCGTA451.8855117E-645.9117338
CGAGACT11400.045.13300323
TCGTTTG851.8189894E-1244.7750740
ACGAGAC11600.044.3441822
ATACACA11950.043.860694
TGTATCG405.628785E-542.268976
TATGCCG3750.041.82727443
TACACAT12900.041.4170385
CGTATGC3650.040.87092641
CCCACGA12650.040.64632819
GAGACTC12950.039.74293524
ATCTCCG13550.039.17833310
CCACGAG13050.038.88437320
Produced by FastQC (version 0.11.5)