FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757513

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757513
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1518739
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT32390.21326903437654526No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT8800.042.1422161
CGACGCT555.455715E-639.3420664
AGGGGGG13300.038.9911370
CTTATAC13900.038.9184761
TCGTATG10150.038.56243540
GGTATCA34400.038.177051
GTATCAA75150.037.939231
TATACAC14900.037.208843
ACGTAGA13850.036.661627
ACGAGAC14550.036.25644722
CCGTCTT9800.036.13934347
TTATACA15200.035.803152
CTCGTAT8100.035.4290539
TCTCGTA8000.035.34744638
CGTAGAG14550.034.91623728
CGTATGC11250.034.85616341
TATCGTA1400.034.4853138
AGACGTA15000.034.0544725
CCCACGA15100.034.02306719
TCTCCGA15800.034.0141111
Produced by FastQC (version 0.11.5)