FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757517

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757517
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1352741
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATGCTTACCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT41290.30523211760418295No Hit
AGTCAGGACATTGCCAATTATTTAGCCTGGTTTCAGCAGAAACCAGGGCAGGCCCCTAGGTCCCTGATCTATG33510.24771926037578515No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT31860.23552180350857999No Hit
GCATTATACTGTTGGCAGTAATAAATTGCAAGATCTTCAGGTTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC31690.2342650958313528No Hit
GATCTATGCTGCGTCCAGTTTGCAAAGTGGGGTCCCATCAAAGTTCAGCGGCAGTGGATCTGGGACAGATTTCA24610.18192691727389057No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21430.1584190913116406No Hit
AGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTC20270.14984390951409027No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19540.14444745890011465No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19070.14097303179248652No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18720.13838569245701876No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17410.12870165094426797No Hit
GTTGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGACATTGCCAATTATTTAGCCTGGTTTCAGCAGAAA16680.12330520033029235No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16270.1202743171087444No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16270.1202743171087444No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC16120.11916545739354392No Hit
GCATAGATCAGGGACCTAGGGGCCTGCCCTGGTTTCTGCTGAAACCAGGC15910.11761305379226328No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14570.10770724033647239No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA45700.041.0685041
GGTATCA17600.040.894251
GTGGTAT6200.038.325341
TGGTATC6450.036.295212
TATCAAC52900.035.598512
TCAACGC55750.034.149194
ATCAACG55450.034.1476863
CAACGCA57100.033.40215
AACGCAG57900.032.940596
ACGCAGA61950.030.559157
CGCAGAG62300.030.3344618
AGAGTAC63400.029.97214711
CAGAGTA64600.029.41538810
GCAGAGT65000.029.0754899
AGTACGG43200.026.29651613
GAGTACG43700.025.99564212
ACGGGTT1359.660653E-925.49776616
ACGAGAC2400.024.39125622
GAGTACT31400.024.22873112
AGTACTT31650.023.81981813
Produced by FastQC (version 0.11.5)