FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005757518

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757518
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1352741
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATGCTTACCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT38640.28564226263564124No Hit
GCATTATACTGTTGGCAGTAATAAATTGCAAGATCTTCAGGTTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC31290.2313081365908182No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT27790.20543474323614055No Hit
AGTCAGGACATTGCCAATTATTTAGCCTGGTTTCAGCAGAAACCAGGGCAGGCCCCTAGGTCCCTGATCTATG27370.2023299360335792No Hit
GATCTATGCTGCGTCCAGTTTGCAAAGTGGGGTCCCATCAAAGTTCAGCGGCAGTGGATCTGGGACAGATTTCA23970.17719578248903523No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21130.15620137188123964No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17710.13091937037466891No Hit
AGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTC17240.12744494326704078No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16680.12330520033029235No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16670.12323127634927898No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15860.11724343388719644No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14020.10364142138073733No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC13580.1003887662161493No Hit
GTTGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGACATTGCCAATTATTTAGCCTGGTTTCAGCAGAAA13570.10031484223513591No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)